產品介紹

Reverse Transcriptase (RT)

FIREScript Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase. This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37℃-50℃. FIREScript RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

SOLIScript Reverse Transcriptase (RT) is an in silico designed chimeric RNA‐directed DNA polymerase. SOLIScript RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60℃). SOLIScript RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.

商品內容

產品特色

1. 室溫下可至少穩定保存一個月、4℃可至少穩定保存六個月，不影響酵素活性。

2. 具有多種包裝型式，可以需求做選擇。

保存條件

-20℃可長期保存，多次凍融不會影響活性。如需經常使用，可存放於4℃

選擇建議表

Series	Name	Number of tubes	Reagents provided	Reagents supplied by the user	Working temperature	cDNA synthesis time*	Feature
FIREScript	RT cDNA Synthesis Mix	3	1. FIREScript Enzyme mix（RT+ RNase Inhibitor） 2. 10x RT Reaction Premix with or without primers 3. Water（Nuclease Free）	1. RNA template 2. gene specific primers	50℃	35 mins	1. Fastest reaction set-up 2. Minimal pipetting
	RT cDNA Synthesis Kit	7	1. FIREScript RT 2. RiboGrip RNase Inhibitor 3.10x RT Reaction Buffer with DTT 4. 20 mM dNTP 5. Oligo dT Primer 6. Random Primers 7. Water（Nuclease Free）	1. RNA template 2. gene specific primers			Flexible reaction composition
	RT Kit	2	1. FIREScript RT 2.10x RT Reaction Buffer with DTT	1. RNA template 2. RNase Inhibitor 3. Oligo dT Primer 4. Random Primers 5. gene specific primers 6. dNTP 7. Water（Nuclease Free）			Economical choice
SOLIScript	RT cDNA Synthesis Mix	3	1. FIREScript Enzyme mix（RT+ RNase Inhibitor） 2.10x RT Reaction Premix with or without primers 3. Water（Nuclease Free）	1. RNA template 2. gene specific primers	60℃		1. Fastest reaction set-up 2. Minimal pipetting
	RT cDNA Synthesis Kit	6	1. FIREScript RT 2. RiboGrip RNase Inhibitor 3.10x RT Reaction Buffer with DTT 4. 20 mM dNTP 5. Oligo dT Primer 6. Random Primers 7. Water（Nuclease Free）	1. RNA template 2. gene specific primers			Flexible reaction composition
	RT Kit	2	1. FIREScript RT 2. 10x RT Reaction Buffer with DTT	1. RNA template 2. RNase Inhibitor 3. Oligo dT Primer 4. Random Primers 5. gene specific primers 6. dNTP 7. Water（Nuclease Free）			Economical choice

訂購資訊

貨號	品名	包裝	Data sheet	MSDS	CoA
06-17-00100	FIREScript RT cDNA synthesis Mix without primers	100 rxn
06-17-00500	FIREScript RT cDNA synthesis Mix without primers	500 rxn
06-18-00100	FIREScript RT cDNA synthesis Mix with Oligo (dT) primers	100 rxn
06-18-00500		500 rxn
06-19-00100	FIREScript RT cDNA synthesis Mix with Random primers	100 rxn
06-19-00500	FIREScript RT cDNA synthesis Mix with Random primers	500 rxn
06-20-00100	FIREScript RT cDNA synthesis Mix with Oligo (dT) and Random primers	100 rxn
06-20-00500		500 rxn
06-15-00050	FIREScript RT cDNA Synthesis Kit	50 rxn
06-15-00200	FIREScript RT cDNA Synthesis Kit	200 rxn
06-13-00050	FIREScript Kit	50 rxn
06-13-00200	FIREScript Kit	200 rxn
06-37-00100	SOLIScript RT cDNA synthesis Mix	100 rxn
06-37-00500	SOLIScript RT cDNA synthesis Mix	500 rxn
06-35-00050	SOLIScript RT cDNA Synthesis Kit	50 rxn
06-35-00200	SOLIScript RT cDNA Synthesis Kit	200 rxn
06-33-00050	SOLIScript Kit	50 rxn
06-33-00200	SOLIScript Kit	200 rxn

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分子生物

PCR

DNA

RNA